Agilent 6490 Triple Quadrupole LC-MS/MS System with 1260 Infinity II HPLC DAD Front-End

Insights by AMPTIUS | AMP Tech Instruments

Ultra-Sensitive Quantitative Mass Spectrometry with Advanced Chromatographic Detection

If your laboratory is struggling to hit ultra-low detection limits or manage dense, unpredictable sample matrices, the Agilent 6490 Triple Quadrupole LC-MS/MS system with a 1260 Infinity II HPLC DAD front-end delivers the ultimate multi-detector powerhouse for trace-level quantification. Powered by innovative iFunnel technology and hexabore capillary ion optics, this high-performance mass spectrometer maximizes ion transmission while stripping away chemical noise to achieve an incredible six orders of linear dynamic range and sub-picogram detection limits. The integrated Agilent 1260 Infinity II Diode Array Detector (DAD) adds a crucial layer of analytical confidence, simultaneously acquiring full-spectrum UV-Vis data from 190–950 nm for real-time peak purity assessment and compound confirmation alongside high-speed Multiple Reaction Monitoring (MRM) acquisition. It is a premier, robust setup for high-throughput environmental labs measuring emerging contaminants like PFAS, as well as regulated pharmaceutical facilities running demanding bioanalysis, drug metabolism, and impurity profiling workflows.

Agilent 6490 LC-MS/MS with 1260 HPLC DAD Front End Price and Cost of Ownership: Reliable Performance at Lower Cost

Certified refurbished systems typically range from approximately $125,000 to $175,000, representing significant savings compared to comparable new systems such as the Agilent 6475 LC-MS/MS paired with a 1260 Infinity II DAD configuration, which commonly range from approximately $350,000 to $420,000 depending on software, detector options, and application-specific accessories. In addition to the initial acquisition cost, laboratories should budget for ongoing operational expenses, including LC columns, solvents, calibration standards, sample preparation supplies, pump seals, autosampler consumables, DAD lamp replacement, nitrogen gas generation or supply, vacuum pump maintenance, ion source cleaning, capillary and ion optics replacement, software support, preventive maintenance, and compliance-related qualification services. Long-term ownership considerations may also include detector servicing, vacuum system maintenance, performance verification, and service agreements designed to maximize uptime and analytical consistency. Purchasing a certified refurbished Agilent 6490 Triple Quadrupole LC-MS/MS System with a 1260 Infinity II DAD front-end enables laboratories to substantially reduce capital expenditures while maintaining access to exceptional MRM sensitivity, robust chromatographic performance, spectral confirmation capabilities, and proven analytical reliability for applications such as PFAS testing, pharmaceutical bioanalysis, pesticide residue monitoring, food safety analysis, environmental contaminant testing, and targeted quantitative research.

EPA Method 8321B: Solvent-Extractable Nonvolatile Compounds Using the Agilent 6490 Triple Quadrupole LC-MS/MS with 1260 Infinity II HPLC DAD Front-End

The Agilent 6490 Triple Quadrupole LC-MS/MS System with an Agilent 1260 Infinity II HPLC System and Diode Array Detector (DAD) front-end provides a powerful platform for laboratories developing and validating workflows related to EPA Method 8321B, which addresses solvent-extractable nonvolatile compounds in environmental samples. The 1260 Infinity II LC provides precise solvent delivery, stable gradient formation, and reproducible chromatographic separation for diverse analyte classes, including phenoxy acid herbicides, organophosphorus compounds, carbamates, dyes, and other nonvolatile organic contaminants. The Agilent 6490 Triple Quadrupole MS provides highly selective quantitative analysis through Multiple Reaction Monitoring (MRM), offering strong sensitivity and reduced matrix interference for trace-level measurements when appropriate LC-MS/MS methods are validated for the target compounds. The integrated DAD provides complementary UV-Visible detection and spectral information, supporting chromatographic monitoring, peak purity assessment, compound characterization, and method development. Because EPA Method 8321B specifies HPLC with thermospray mass spectrometry (HPLC/TS-MS) or UV detection, laboratories using the 6490 ESI-based LC-MS/MS platform should establish and validate the applicable analytical procedure rather than treating the instrument as a direct thermospray-MS implementation of the method. Combining high-performance liquid chromatography, selective triple-quadrupole mass detection, and complementary DAD analysis, the Agilent 6490/1260 Infinity II configuration provides a flexible solution for environmental laboratories performing validated quantitative analysis of nonvolatile organic contaminants in water, soil, sediment, and waste matrices.

AMP Tech Analytical Solutions for Academic Research and Grant-Funded Laboratories

At AMPTIUS | AMP Tech Instruments, we support the advancement of scientific education and innovation by providing cost-effective analytical instruments tailored to the unique budget constraints of the academic sector. Every pre-owned system in our inventory is independently evaluated and fully verified within our in-house testing laboratory, making our AMP Tech Certified equipment an ideal choice for university chemistry departments, core facilities, and researchers optimizing limited grant funding. Because academic applications vary heavily from undergraduate teaching labs to complex graduate research, we precisely document each unique listing to ensure the specific components align with your educational goals or specific institutional SOPs. To guarantee that your system includes the exact module configuration required for your research methodology and to align with your university’s procurement process or funding timeline, we highly encourage you to connect directly with our active sales management team or request a customized quote before purchase.