Agilent 1100 HPLC with FLD and Quaternary Pump

Insights by AMPTIUS | AMP Tech Instruments

Agilent 1100 HPLC with FLD: High-Sensitivity Fluorescence Analysis Across Key Industries

If your laboratory is struggling to hit low detection limits for trace-level target compounds or manage complex sample backgrounds, the Agilent 1100 HPLC system equipped with a Fluorescence Detector (FLD) delivers the extreme sensitivity and selectivity your assays demand. Driven by a robust Agilent 1100 Quaternary Pump, this system accurately blends up to four mobile phases at flow rates up to 10 mL/min, simplifying your multi-solvent method development while ensuring stable gradient delivery. The high-sensitivity fluorescence detector utilizes a powerful xenon flash lamp with programmable excitation and emission wavelengths from 200 to 650 nm, supporting simultaneous multi-signal acquisition to monitor multiple wavelength combinations in a single run. It is an industry-standard, cost-effective workhorse for environmental labs tracking trace polycyclic aromatic hydrocarbons (PAHs), food safety facilities conducting routine aflatoxin or vitamin screenings, and pharmaceutical operations running demanding impurity profiling.

Agilent 1100 HPLC FLD System Price and Cost of Ownership: Reliable Performance at Lower Cost

The Agilent 1100 HPLC System with a Fluorescence Detector (FLD) and Quaternary Pump provides an economical and highly sensitive liquid chromatography solution for environmental, food safety, pharmaceutical, biotechnology, and research laboratories. Certified refurbished systems typically range from approximately $12,000 to $14,500, offering substantial savings compared to comparable new systems such as an Agilent 1260 Infinity II Prime LC equipped with an InfinityLab FLD and Quaternary Flex Pump, which commonly range from approximately $42,000 to $51,000 or more, depending on configuration and software options. Beyond the initial acquisition cost, laboratories should account for ongoing operational expenses, including HPLC columns, solvents, mobile phase preparation, pump seals, check valves, autosampler maintenance, fluorescence lamp replacement, calibration standards, software support, preventive maintenance, and compliance-related qualification services. Long-term ownership costs remain manageable due to the proven reliability of the Agilent 1100 platform, widespread availability of replacement parts, and straightforward serviceability. Purchasing a certified refurbished Agilent 1100 HPLC with FLD and Quaternary Pump allows laboratories to significantly reduce capital expenditures while benefiting from highly sensitive fluorescence detection, flexible method development capabilities, dependable chromatographic performance, and proven analytical reliability for applications such as PAH analysis, mycotoxin testing, pharmaceutical impurity analysis, amino acid determination, and trace-level contaminant monitoring.

EPA Method 8310: Polynuclear Aromatic Hydrocarbons Using the Agilent HPLC 1100 Series Fluorescence Detector and Quaternary Pump

The Agilent 1100 HPLC System with Fluorescence Detector (FLD) and Quaternary Pump is well suited for analytical workflows aligned with EPA Method 8310 for the determination of polynuclear aromatic hydrocarbons (PAHs) in environmental matrices such as soil, sediment, and solid waste extracts. Because many of the target PAHs exhibit strong native fluorescence, the FLD provides sensitive and selective detection without requiring derivatization, making it particularly effective for trace-level PAH analysis. The quaternary pump provides precise mobile-phase delivery and gradient control to support reproducible chromatographic separation of complex PAH mixtures. When appropriately configured, calibrated, and validated against laboratory SOPs and the applicable method requirements, the Agilent 1100 platform can support EPA 8310 workflows while providing reliable retention-time reproducibility, sensitive fluorescence detection, and consistent quantitative performance. This configuration is particularly valuable for environmental laboratories performing routine PAH monitoring, contaminated-site assessment, wastewater and sediment analysis, and regulatory testing where selective fluorescence-based detection is advantageous.

AMP Tech Analytical Solutions for Academic Research and Grant-Funded Laboratories

At AMPTIUS | AMP Tech Instruments, we support the advancement of scientific education and innovation by providing cost-effective analytical instruments tailored to the unique budget constraints of the academic sector. Every pre-owned system in our inventory is independently evaluated and fully verified within our in-house testing laboratory, making our AMP Tech Certified equipment an ideal choice for university chemistry departments, core facilities, and researchers optimizing limited grant funding. Because academic applications vary heavily from undergraduate teaching labs to complex graduate research, we precisely document each unique listing to ensure the specific components align with your educational goals or specific institutional SOPs. To guarantee that your system includes the exact module configuration required for your research methodology and to align with your university’s procurement process or funding timeline, we highly encourage you to connect directly with our active sales management team or request a customized quote before purchase.